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Role of post-transcriptional gene silencing in spermatogenesis in mice.
Male germ cell maturation is a complex process, which involves the concerted action of numerous proteins, and gene and protein regulations are integrated to control spermatogenesis. Post-transcriptional gene silencing is an intrinsic quality of cells which expresses homologous genes, and it is an important process in other biological processes such as development, cell cycle and infection. However, the function of post-transcriptional gene silencing in spermatogenesis remains unknown. Here, we compared the expression patterns of genes and proteins in adult and neonatal mice testis, and the expression patterns of genes and proteins in mouse testis with post-transcriptional gene silencing and without post-transcriptional gene silencing by retroviral-mediated shRNA expression. In addition, spermatogenesis was characterized by the expression patterns of genes and proteins in testicular germ cells. Our studies demonstrate that the post-transcriptional gene silencing contributes to spermatogenesis by regulating genes and proteins. We observed that many testis genes are down-regulated, and proteins are silenced in mouse testis after retroviral-mediated shRNA expression. For the first time, we showed that post-transcriptional gene silencing negatively regulates genes and proteins involved in development, cell cycle and apoptosis in mouse testis. This is the first evidence for the role of post-transcriptional gene silencing in spermatogenesis in mice.The Syrian army has advanced into the Islamic State-held city of Falluja, Islamic State said, hours after the extremist group released an audio message purportedly from its leader, Abu Bakr al-Baghdadi.

“The gates of Islam is opening in this blessed city,” said Abu Mohammad al-Adnani, the head of the IS’s external operations in its self-declared caliphate, claiming that Islamic State had recaptured “a large part” of the city. “God willing, we will return Falluja to its former glory.”

His statement was sent in a Twitter message https://theblinkapp.com/wp-content/uploads/2022/06/MaxDIR.pdf

 

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